Part:BBa_K5299008:Design
BG37: Autoinducible promoter in exponential phase
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Zobel et al. identified the BG synthetic promoters by systematically analyzing promoter activities in E. coli and Pseudomonas strains, particularly P. aeruginosa and P. putida. They found that the consensus sequences, especially the −10 and −35 regions, closely resembled those of sigma-70 promoters in E. coli, which suggested similar transcriptional mechanisms across these species. Using an initial plasmid-based selection in E. coli PIR2 cells, they efficiently screened for effective synthetic promoters, confirming their comparable activity in both E. coli and Pseudomonas [1].
Source
We ordered the sequence of the BG37 promoter combined with the RBS BBa_B0034 from IDT, incorporating Golden Braid overhangs. The BG37 sequence was identified in the work of Zobel et al. (2015), while the RBS corresponds to the BBa_B0030 sequence from the registry.
References
[1] Zobel, S., Benedetti, I., Eisenbach, L., de Lorenzo, V., Wierckx, N., & Blank, L. M. (2015). Tn7-Based Device for Calibrated Heterologous Gene Expression in Pseudomonas putida. ACS synthetic biology, 4(12), 1341–1351. https://doi.org/10.1021/acssynbio.5b00058